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KMID : 0379119990270010020
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Korean Journal of Mycology
1999 Volume.27 No. 1 p.20 ~ p.26
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The Specific Probes Confirming the Genomic DNA of Tricholoma matsutake in Korea
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Lee Sang-Sun
Hong Sung-Woon
Chung Hung-Chae
Sung Chang-Kun
Kim Jae-Hun
Ka Kang-Hyeon
Kim Hyun-Joong
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Abstract
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The specific DNA band appeared in PCR-RAPD analysis using OPO-2 primer was a very important for the researching Korean pine-mushrooms, Tricholoma matsutake. This DNA band, sequenced to be the 770 base pairs, existed as only a single copy in the whole genomic DNA´s of Korean pine-mushrooms. However, this band was not presenting from the PCR-RAPD bands of other ectomycorrhyzal fungi reacted with the OPO-2 primer or the dot blots. Also, this DNA sequence was not matched with those of the other genes known by NCBI and had low homology together with sequence of other proteins compared. Those results suggested that the specific DNA band can be used as probe for identification of T. matsutake and might be related to the informations rather than the gene for the proteins with analysis of protein sequence translated from the DNA sequence.
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KEYWORD
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